DNA/RNA Biology, RT/PCR/qPCR & NGS
We offer an all inclusive range of individual reagents and kits that enable the preparation and quantitation of RNA/miRNA/lncRNA and DNA for subsequent application(s) from reverse transcription, end-point and real-time PCR to next generation sequencing and library construction.
Search in our product collection or ask for your tailored package.
New: Simplify Your DNA or RNA Extraction
Traditional DNA and RNA extraction methods often rely on proteinase K, SDS, and purification by columns, beads, or solvent extraction. Extraneous agents are added that subsequently need to be removed – all in an effort to produce high-quality nucleic acids.
Much of the problem is the inadequacy of the lysis step. Proteinase K doesn’t work at a high enough temperature to denature the proteins it is digesting. To compensate, SDS is added but is toxic to every downstream diagnostic. So it must be removed, initiating a cascade of steps – each solving what was done in the previous step.
MicroGEM extraction solutions extraction solutions offers specially-formulated cocktails of thermophilic and mesophilic enzymes and buffers work at different temperatures, creating coordinated temperature-driven reactions easily done using a thermal cycler or MicroGEM’s PDQeX Nucleic Acid Extractor.
This simplified workflow produces high-quality DNA and RNA from a single cell or from thousands of cells – all in minutes, not hours – with good yields, less chance of error, and reduced risk of cross-contamination.
DNA, RNA & miRNA Isolation
Isolation of DNA, RNA and miRNA from tissues, mammalian cells, bacteria, plants and difficult sample types like serum, FFPE material, saliva, soil, stool, urine or milk is an easy task when using our kits. A selection of these kits are available also in a 96-well format. It is worth noting that these kits ensure high qualitative results at very competitive
Ethidium Bromid Destroyer
EtBr Destroyer is a specifically designed reagent that effectively degrade and destroy the EtBr and result in a non-fluorescence and non-mutagenic remainder. It is provided in two different formats, as a spray (for the treatment of clean surfaces and solid waste) or as powder (to decontaminate liquid waste).
NGS Kits, Enzymes & Services
The currently available Next-generation sequencing technologies revolutionize research in life science (especially genomics) and medical applications. NGS technologies include a number of methods for template preparation, sequencing and imaging, as well as data analysis. However, the basic sample process requirements, so called „library construction“, are shared among all of the technology platforms like Illumina and IonTorrent.
We offer an all-inclusive range of individual enzymes (Phi29 DNA polymerase, T4 RNA ligase), DNA purification and NGS library preparation kits (e.g. RapidSeq-Series for DNA or RNA) to allow fast and cost-effective NGS sequencing.
CancerSeq™ NGS Characterized Tissues are high quality genetically characterized tissues from real tumors that are ideal for verification, genotyping, and identification of new mutational hotspots.
ABMgood is an Illumina Certified Service Provider, dedicated to ensuring the delivery of highest-quality data available for genetic analysis applications.
DI Dr. Chantal Rodgarkia
+43 664 968 29 70
Cloning Kits & Restriction Enzymes
Besides classical cloning methods with restriction enzymes and ligases, we also offer alternative cloning methods.
The EZShuttle™ LR Recombination Cloning System uses a site-specific recombination machinery between E. coli and phage lambda genomes for efficient DNA fragment transfer between plasmids, the same principle as Gateway® technology.
Fast-Fusion™ Cloning and Ligation-Free Cloning provide a rapid method for assembling your PCR product(s) into a linearized vector in just 15 minutes. The progressive design has eliminated the requirement for compatible ends and unique restriction sites within the vector and inserts.
Search for restriction enzymes, ligases, DNA/RNA modifiying enzymes and cloning kits in our database.
Reverse Transcription, DNA Polymerases & qPCR Products
We offer a wide range of PCR, RT-PCR and qPCR products including enzymes. These products are partially certified (ISO 13485) and available as kits or ready-to-use mastermixes.
Robust cDNA synthesis from any RNA template, high reproducibility and excellent yield are a prerequesite when working with a small number of cells.
Explore our collection of PCR, RT-PCR and qPCR products in our database. The following product lines can be of certain importance for your experiments:
PCR directly from blood samples can be done with Bloodirect DNA Polymerase. Ask for your free sample using the following sample code: ”Vampires do PCR” .
Guaranteed high-performance real-time PCR using abm’s BrightGreen qPCR MasterMix. abm’s BrightGreen qPCR MasterMix provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Ask for your sample to convince the lab colleagues and your PI to change to BrightGreen qPCR MasterMix.
Nucleic Acid Quantitation
iQuant™ dyes are highly distinctive between DNA and RNA by fluorimetric quantification with Qubit or Quantus. It allows 100 times more sensitivity than measurements with NanoDrop.
iQuant™ Nucleic Acid Quantitation Selection Guide Target Initial Sample Concentration Application iQuant™ HS dsDNA Quantitation Kit dsDNA 4 pg/µL – 100 ng/µL Quantitation of PCR products, viral DNA, and samples for subcloning and Next Gen Sequencing iQuant™ NGS-HS dsDNA Assay Kit dsDNA 4 pg/µL – 100 ng/µL Quantitation of DNA samples for Next Gen Sequencing iQuant™ BR dsDNA Quantitation Kit dsDNA 40 pg/µL – 1,000 ng/µL Quantitation of genomic and miniprep DNA samples iQuant™ NGS-BR dsDNA Assay Kit dsDNA 40 pg/µL – 1,000 ng/µL Useful for quantitation of DNA samples for Next Gen Sequencing iQuant™ ssDNA Quantitation Kit ssDNA 20 pg/µL – 200 ng/µL Quantitation of oligo, primers, denatured DNA, PCR products iQuant™ HS RNA Quantitation Kit RNA 100 pg/µL – 100 ng/µL Quantitation of samples for microarray, RT-PCR, and Northern blot procedures iQuant™ BR RNA Quantitation Kit RNA 400 pg/µL – 1,000 ng/µL Quantitation of samples for microarray, RT-PCR, and Northern blot procedures
DNA Ladders & DNA Stains
Get rid of ethidium bromide in your lab! We recommend to use EtBr-free alternatives like DNA gel stains and DNA loading dyes from Genecopoeia and ABMgood:
Cat.No Product Format DNA/RNA Sensitivity Visualisation GC-N103 GreenView Ultra 10,000X gel stain; 5000x post-stain dsDNA, ssDNA, RNA Ultra sensitive (mid pg range) Blue light/UV SYBR filter GC-N101 GreenView Plus 10,000X gel stain; 5000x post-stain dsDNA, ssDNA, RNA Highly sensitive (high pg range) Blue light/UV SYBR filter GC-D013 SafeRed Loading Dye 6X loading dye dsDNA Sensitive (low ng range) UV red filter GC-N102 RedView 10,000X gel stain; 5000x post-stain dsDNA, RNA Sensitive (low ng range) UV red filter GC- N100 GreenView 10,000X gel stain; 5000x post-stain dsDNA Sensitive (low ng range) Blue light/UV SYBR filter GC-D012 SafeGreen Loading Dye 6X loading dye dsDNA, ssDNA, RNA Sensitive (low ng range) Blue light/UV SYBR filter
Ask for your free gel stain sample (from Genecopoeia) using the following code: “Let it glow” .
Compare your DNA ladder to 100bp, 1kb or 2kb Opti-DNA Marker, also available in a ready-to-use format premixed with Safe-Green™.