DNA/RNA Biology, RT/PCR/qPCR & NGS
We offer an all inclusive range of individual reagents and kits that enable the preparation and quantitation of RNA/miRNA/lncRNA and DNA for subsequent application(s) from reverse transcription, end-point and real-time PCR to next generation sequencing and library construction.
New: Simplify Your DNA or RNA Extraction
Traditional DNA and RNA extraction methods often rely on proteinase K, SDS, and purification by columns, beads, or solvent extraction. Extraneous agents are added that subsequently need to be removed – all in an effort to produce high-quality nucleic acids.
Much of the problem is the inadequacy of the lysis step. Proteinase K doesn’t work at a high enough temperature to denature the proteins it is digesting. To compensate, SDS is added but is toxic to every downstream diagnostic. So it must be removed, initiating a cascade of steps – each solving what was done in the previous step.
MicroGEM extraction solutions extraction solutions offers specially-formulated cocktails of thermophilic and mesophilic enzymes and buffers work at different temperatures, creating coordinated temperature-driven reactions easily done using a thermal cycler or MicroGEM’s PDQeX Nucleic Acid Extractor.
This simplified workflow produces high-quality DNA and RNA from a single cell or from thousands of cells – all in minutes, not hours – with good yields, less chance of error, and reduced risk of cross-contamination.
DNA, RNA & miRNA Isolation
Isolation of DNA, RNA and miRNA from tissues, mammalian cells, bacteria, plants and difficult sample types like serum, FFPE material, saliva, soil, stool, urine or milk is an easy task when using our kits. 96-well format is also available for a selection of these kits. It is worth noting that these kits ensure high qualitative results at very competitive prices.
Ethidium Bromid Destroyer
EtBr Destroyer is a specifically designed reagent that effectively degrade and destroy the EtBr and result in a non-fluorescence and non-mutagenic remainder. It is provided in two different formats, as a spray (for the treatment of clean surfaces and solid waste) or as powder (to decontaminate liquid waste).
NGS Kits, Enzymes & Services
The currently available Next-generation sequencing technologies revolutionize research in life science (especially genomics) and medical applications. NGS technologies include a number of methods for template preparation, sequencing and imaging, as well as data analysis. However, the basic sample process requirements, so called „library construction“, are shared among all of the technology platforms like Illumina and IonTorrent.
We offer an all-inclusive range of individual enzymes (Phi29 DNA polymerase, T4 RNA ligase), DNA purification and NGS library preparation kits (e.g. RapidSeq-Series for DNA or RNA) to allow fast and cost-effective NGS sequencing.
CancerSeq™ NGS Characterized Tissues are high quality genetically characterized tissues from real tumors that are ideal for verification, genotyping, and identification of new mutational hotspots.
ABMgood is an Illumina Certified Service Provider, dedicated to ensuring the delivery of highest-quality data available for genetic analysis applications.
DI Dr. Chantal Rodgarkia
+43 664 968 29 70
Cloning Kits & Restriction Enzymes
Besides classical cloning methods with restriction enzymes and ligases, we also offer alternative cloning methods.
The EZShuttle™ LR Recombination Cloning System uses a site-specific recombination machinery between E. coli and phage lambda genomes for efficient DNA fragment transfer between plasmids, the same principle as Gateway® technology.
Fast-Fusion™ Cloning and Ligation-Free Cloning provide a rapid method for assembling your PCR product(s) into a linearized vector in just 15 minutes. The progressive design has eliminated the requirement for compatible ends and unique restriction sites within the vector and inserts.
Search for restriction enzymes, ligases, DNA/RNA modifiying enzymes and cloning kits in our database.
Reverse Transcription, DNA Polymerases & qPCR Products
We offer a wide range of PCR, RT-PCR and qPCR products including enzymes. These products are partially certified (ISO 13485) and available as kits or ready-to-use mastermixes.
Robust cDNA synthesis from any RNA template, high reproducibility and excellent yield are a prerequesite when working with a small number of cells.
Explore our collection of PCR, RT-PCR and qPCR products in our database. The following product lines can be of certain importance for your experiments:
Guaranteed high-performance real-time PCR using ABM´s BrightGreen qPCR MasterMix or MedChem Express SYBR Green qPCR Master Mix. These master mix provide all ingredients necessary for quantitative PCR in a premixed and optimized format. Ask for your sample to convince the lab colleagues and your PI to change to BrightGreen qPCR MasterMix (ABM) or SYBR Green qPCR Master Mix (MedChem).
Nucleic Acid Quantitation
ABP Biosciences iQuant™ DNA or RNA Assay Kits allow precise quantitation of DNA or RNA samples across a wide range of concentrations. It allows 100 times more sensitivity than measurements with NanoDrop. Our new iQuant™ NGS dsDNA assay kits are ideal for quantitating DNA samples in Next Gen Sequencing applications. Unlike absorbance-based nucleic acid quantitation, fluorescence-based DNA or RNA quantitation is highly sensitive and selective for DNA or RNA, and provides more accurate DNA or RNA concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts.
- iQuant™ dsDNA HS Assay Kit
- iQuant™ dsDNA BR Assay Kit
- iQuant™ ssDNA Assay Kit
- iQuant™ RNA HS Assay Kit
- iQuant™ RNA BR Assay Kit
- iQuant™ NGS-HS dsDNA Assay Kit
- iQuant™ Assay Tubes
DNA Ladders & DNA Stains
Developed to replace toxic Ethidium bromide (EtBr), SafeView™ products from ABM are a new class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in both agarose and polyacrylamide gels. Six formulations are available, for compatibility with any experimental setup.
ABP Biosciences offers next-generation nucleic acid gel stains for pre- and post-gel staining, and for mixing with nucleic acids prior to electrophoresis. These stains are fluorescent nucleic acid gel stains designed to replace the highly mutagenic ethidium bromide (EB).