THP Medical Products

Pancreatic Elastase

Two ELISA test kits (based on monoclonal antibodies) are available for the determination of pancreatic elastase 1. The stool test quantifies E1 in stool, allowing the diagnosis or exclusion of pancreatic exocrine insufficiency, which can be caused by chronic pancreatitis, cystic fibrosis, pancreatic tumor, cholelithiasis or diabetes mellitus for example.

The serum test quantifies E1 in serum, allowing the diagnosis or exclusion of an acute pancreatitis or an inflammatory episode of chronic pancreatitis or ERCP- or gallstone-induced pancreatitis.

M2-PK

M2-PK detects a metabolic state highly specific for a variety of tumors. Its superior usefulness was demonstrated in diagnosis and monitoring of different cancer types. It’s the first reliable tumor marker for renal cell carcinoma.

The majority of human tumors strongly overexpress an isoform of the glycolytic enzyme pyruvate kinase, the type M2. This isoenzyme is released from tumor cells and is quantitatively detectable in body fluids. The concentration of the type M2 isoenzyme indicates a metabolic switch turning normal cells into tumor cells. It highly correlates with the malignancy of cancer (staging) and it is independent from the histological grading.

This novel type of marker for malignancies is called M2-PK and is the only metabolic marker so far. ScheBo® • Biotech AG developed a highly sensitive enzyme-linked immunosorbent assay (ELISA) which allows the quantitative measurement of M2-PK in EDTA plasma and stool.

The M2-PK™ Plasma Test is based on two monoclonal antibodies which specifically react with M2-PK and do not cross react with the other isoforms of pyruvate kinase (Typ L, R, M1 and M2). As M2-PK is a highly tumor specific protein and shows no organ specificity it may be the marker of choice for a variety of tumors.

The ScheBo® •  M2-PK™ Stool Test is a totally new approach for bowel cancer screening tests. Previously, only non-specific tests for blood in the stool could be used to give an indication of an existing bowel cancer or its precursors. With the new ELISA method for M2-PK in the stool it′s now possible to detect bleeding or non-bleeding bowel cancers, as well as polyps, with high sensitivity and specificity. The test is more accurate because it is not dependent on occult blood.

Cell proliferation and viability analysis is crucial for cell growth and differentiation studies and is often used to evaluate both compound toxicity and inhibition of tumor cell growth during drug development. Proliferation measurements are typically made based on average DNA content or on cellular metabolism. Assays can report either total cell numbers or live cells, or measure DNA synthesis in single cells. Viability assays measure the population of dead and live cells from total cell numbers. The most prominent assays are MTT, MTS, XTT, WST, Alamar Blue, BrdU, EdU and CFSE, calcein AM, Annexin V binding, JC-1, TUNEL assay and Caspase activity assays.

Please use our product search to find further information on these assays.

Cellular metabolism is a process that involves a number of enzyme catalyzed reactions that facilitate digestion and transport of components between cells that help them to sustain, grow and perform its cellular functions. Our collection of assays is diverse and includes assays for glycerol, citrate, glucose, ATP/ADP, glutathione  and many more. The assays are high throughput-adaptable, easy-to-use, sensitive and compatible with a variety of sample types.

Oxidative stress is a state when the normal reduced environment of a cell changes due to the accumulation of reactive oxygen species (ROS). These ROS like peroxides, superoxides and free radicals can cause pathological changes in the cell due to DNA damage, oxidation of amino acids, proteins, polyunsaturated fatty acids and enzyme cofactors which subsequently causes cell death due to apoptosis or necrosis. Our collection of assays are include assays for lipid peroxidation, oxidized LDL, protein oxidation and nitration, DNA and RNA damage, Comet Assay, myeloperoxidase, NOS, HNE and many more.

Cell adhesion is a complex mechanism involved in a variety of processes including cell migration & invasion, embryogenesis, wound healing and tissue remodeling and involves extracellular matrix (ECM) components like fibronectin, collagen and more.

Cell migration is a highly integrated, multi-step process that plays an important role in the progression of various diseases including cancer, atherosclerosis and arthritis. There are various types and definitions of cell migration.

Transformation of normal cells into neoplastic cells occurs via a series of genetic and epigenetic alterations, yielding a cell population capable of proliferation independently of both internal and external signals that normally restrain their growth. Traditionally the soft agar colony formation assay has been used to monitor cell transformation and anchorage-independent growth, with manual counting of proliferated cells after 3-4 weeks of cell growth.

For the most standardized and highly reproducable assays check our collection.